菲降解菌的降解特性及酶促反应动力学研究

通过对取自MBR膜生物反应器中的活性污泥加入菲进行富集培养、驯化,分离、纯化出一株能以菲为唯一碳源和能源的短杆状革兰氏阴性菌J-1,细菌长2～5μm,宽1～3μm;研究了初始底物浓度、温度、pH对菌株J-1降解菲的影响,探讨了菌株J-1胞内酶对菲降解的底物抑制动力学。试验表明:菌株J-1在48h内能将不同浓度菲的水溶液中的菲完全降解;菲浓度增加,达到完全降解的时间延长。温度对细菌的降解能力影响较大,菌株J-1对菲降解的最佳温度为28℃。1.15mg·L-1的菲,28℃时48h内能完全降解,而相同时间内10℃时的降解率仅为36.65%。菌株J-1对pH的波动具有一定的适应性,pH在一定范围内(6.0～8.4)变化对菲降解的影响不大,降解反应的最佳pH为7.2。菌株J-1对菲的降解符合一级动力学反应方程。较高的底物浓度对酶促降解反应具有抑制作用,酶促反应的最大速率常数vm=1.17mg·L-1·h-1,米氏常数Km=61.70mg·L-1;底物抑制常数kS=49.60mg·L-1;最佳底物浓度[S]opt=55.32mg·L-1。     

福建石炭纪岩相古地理分析

笔者总结了福建省石炭纪生物地层与岩石地层的研究成果,提出了生物地层与岩石地层的划分对比。在详尽的相分析基础上对该纪地层进行了相系、相、亚相及微相的划分,并按“优势相”的原则分期编制了林地期、黄龙期及船山期的岩相古地理图。笔者采用“相、层、位”控矿观点对石炭纪的矿产成因及成矿规律作了探讨。     

19802000北京市农业土地生产性的变动分析

根据1980、1990、2000年的统计资料和实地走访观察,本文分析了北京市乡镇一级的粮食作物、蔬菜作物和果品作物的分布格局及农业土地生产性近20年的变化。研究认为:1980年北京农业生产分布格局与土地生产性为比较典型的杜能模式;随着农业经营的多样化,1990年和2000年北京农业土地生产性呈现出多样的分布格局;北京粮食作物、蔬菜作物、果品作物的分布格局及农业土地生产性近20年的变化与此阶段的农业生产政策、农业以外的社会经济活动变化有较强的关联性;对北京以上问题的研究为验证杜能、辛克利亚、布莱昂特城市周边农业地域研究经典理论的演变提供了实证案例。     

Uniaxial compression and tension tests of anthracite and loading rate dependence of peak strength

The mechanical characteristics of coal under uniaxial compressive stress and uniaxial tensile stress are key factors in investigations of the stability of galleries and coal faces, and are vital considerations for the efficient design of coalmines, disaster prevention, and environmental preservation. These mechanical characteristics have long been a topic of research, but remain insufficiently understood. In this study we performed uniaxial compression tests and uniaxial tension tests on coal, with particular attention to two concerns. The first was to measure the loading rate dependence of the peak strength of coal. Coal shows high sample-to-sample scatter in strength; test methods that are successful in comparatively homogeneous rock types cannot be used for coal. We therefore used an alternative test method that was recently developed by the authors. Anthracite samples were subjected to alternating slow and fast strain rates. Measured variations in stress during this process were used to estimate the loading rate dependence of peak strength. The second objective was to obtain complete stress–strain curves for coal under uniaxial tensile stress. It is difficult to hold samples secure during a tensile test; consequently, such curves have yet to be obtained. This study presents a successful application of the authors' method to the analysis of coal samples, yielding a complete stress–strain curve under tensile stress. The two methods presented here hold promise for application not only to anthracite but also to a wide variety of coals. It is possible to derive the values of the constants used in constitutive equations from the obtained experimental results. Once these equations are determined, they can be incorporated into finite-element software to investigate various time-dependent behaviors of coal and aid in the efficient design of coalmines and the prevention of subsidence and mine disasters.     

Exploiting the dual functions of polymer depressants in fine particle flotation

Fine hydrophilic particles are known to be entrained with water in flotation of many ores. Flocculation of the hydrophilic particles by polymer depressants could potentially reduce the mechanical entrainment of these particles. This paper reports testwork completed on fine particles of several solids, iron oxide, hydroxyapatite and sphalerite, as well as on a relatively coarse quartz sample (− 75 + 38 μm). Dodecylamine was used as a collector for quartz, and several dispersants and polymer depressants, including sodium silicate, sodium metaphosphate, zinc sulfate, cornstarch, corn dextrin and carboxymethyl celluloses (with molecular weights of both 700,000 and 80,000) were used as flotation modifiers. The major part of the testwork involved flotation tests in a 200 mL flotation column. It was observed that flocculation of the fine hydrophilic particles significantly reduced their mechanical entrainment, while dispersion severely aggravated it. Thus, in the flotation separation of synthetic mixtures of the − 75 + 38 μm quartz and fine (reagent grade) iron oxide or hydroxyapatite, polymer depressants that caused flocculation performed better than those that did not cause flocculation.     

Effect of some additives on synthesis of zeolite from coal fly ash

Hydrothermal conversion of fly ash into zeolites was conducted and the effects of the addition of sodium halide and waste solutions produced after zeolitization of fly ash, as well as the adjustment of the Si/Al ratio prior to synthesis process on the formation and cation exchange capacity (CEC) of zeolite product were evaluated. Both the addition of NaCl and NaF ameliorated the crystallinity and CEC of synthesized zeolite, but NaF had a better improvement effect. Na⁺ was considered to enhance the crystallization of zeolite, while F⁻ favored the dissolution of fly ash. The type of zeolite formed depended on the Si/Al ratio of the starting material prior to the nucleation and crystallization of zeolite. The adjustment of the Si/Al ratio of fly ash by addition of Na₂SiO₄ and Al(OH)₃ changed the type and CEC of zeolite. Waste solutions contained large amount of Si and little Al due to the formation of a zeolite named NaP1 in zeolite terminology with the Joint Committee of Powder Diffraction Standard (JCPDS) code of 39-0219. The alkalinity decreased largely. As a result, the CEC value of zeolite products synthesized with waste solution as alkali source decreased. The supplementation of new alkali to adjust the alkalinity of waste solution could enhance the CEC of synthesized product. It was concluded that: (1) addition of sodium halide and adjustment of the Si/Al ratio prior to synthesis can improve the quality of zeolite; (2) waste solutions produced following the zeolitization of fly ash can be reused as an alkali source in the activation of fly ash; zero-emission of waste solution in the synthesis of zeolite from fly ash is possible.     

贵州南部“半边街式”铅锌矿床地质特征及其成因探讨

贵州省南部地区是全国重要的汞、锑资源分布区。2000年由于产于上泥盆统中铅锌矿的陆续发现,贵州省地矿局启动了以铅锌资源评价和成矿规律研究为主的地质工作,目标是总结铅锌矿产出的地质特征和成矿规律。通过三年的研究认为,该区处扬子准地台、华南褶皱带和右江造山带交汇部位,受晚古生代初期发生的陆内裂谷活动影响,产生了一个与桂中台陷相连的大型沉积凹陷区—黔南台陷沉积区,形成了巨厚碳酸盐和碎屑岩沉积,产生了与裂陷盆地边缘同沉积断层活动有关的低温成矿作用,造成了在晚泥盆纪以前的地层中汞、锑、砷、金、铅锌等矿床沿同沉积断层密集分布。本文通过矿床特征总结和矿床成因探讨,建立了准同生沉积成矿作用模式,旨在为该区寻找这类矿床提供参考和借鉴作用。     

流动人口分布与区域经济发展关系若干解释(1990、2000)

本文对我国1990、2000年间流动人口在东、中、西部和东部地区中环渤海、长江三角洲、珠江三角洲及福建三个亚区的分布变化以及这一分布变化与上述各地区各相关经济与非经济因素变化的关系进行了研究。研究发现,10年中流动人口东、中、西部分布变化与东、中、西部经济发展的变化高度一致;而10年中流动人口在东部地区环渤海、长江三角洲、珠江三角洲及福建这三个亚区层面的分布变化则与经济发展要素变化有一致的方面,也有不一致的方面。珠江三角洲及福建的经济增长带来了流动人口的大幅增长,除珠江三角洲劳动密集型企业众多外,这可能与该区域地方社会人文等文化本底所决定的经济发展制度及习俗有一定的相关性。     

Adsorption of aluminium by a latosol as affected by phosphate and glutamic acid

The effect of phosphate and glutamic acid on adsorption of aluminium onto a latosol was investigated as a function of pH and ligand concentrations through batch equilibrium experiments. The results showed that adsorption of aluminium by the soil was enhanced after addition of phosphate at low pH, and this promotive effect was gradually eliminated with the increase in pH. The positive effect of phosphate on aluminium adsorption onto latosol was attributed to phosphate-induced surface negative charge and formation of ternary surface complexes involving aluminium and phosphate. While using silica as adsorbent, the inactive surfaces for phosphate, promotion of adsorption of aluminium by phosphate was also found. Then it was proposed that additional aluminium might bind to the phosphate adsorbed onto the silica in the form of surface complexes silica–aluminium–phosphate again, and the mechanism might operate in the soil systems as well. Glutamic acid exhibited no influence on the adsorption of aluminium by the soil at low pH. After increasing the pH, adsorption of aluminium was inhibited especially with the highest addition of glutamic acid, probably owing to weak affinity of complexes between glutamic acid and aluminium to the soil.